Review



human ace2 orf cdna  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Sino Biological human ace2 orf cdna
    O-GlcNAcylation of S at S659 regulates pseudoviral packaging of SARS-CoV-2. ( A ) S-S659A does not affect the binding between S and <t>Ace2.</t> HEK293T cells were transfected with the plasmids expressing HA-ACE2 and GFP-S-WT, GFP-S-659A, or empty vector. The cell lysates were subject to IP. ( B ) Quantitation of ( A ). ( C ) Diagram of four-plasmid system for SARS-CoV-2pp production. The four-plasmid system for preparing pseudovirus SARS-CoV-2pp includes Luc (pLenti6) encoding luciferase, HIV Gag/pol (pLP1), HIV Rev (pLP2), and GFP-S. ( D ) S659A mutation in SARS-CoV-2 S reduced the SARS-CoV2pp package efficiency. The luciferase activity of lysates from Huh7.5.1 cells infected with SARS-CoV-2pp generated from HEK293T cells based on either S or the S-S659A was assayed. ns, no significance; ****, P < 0.0001.
    Human Ace2 Orf Cdna, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+cdna/Human+ACE2+%2F+Angiotensin-Converting+Enzyme+2+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-HA+tag/pmc12403561-103-1-15
    Average 96 stars, based on 2 article reviews
    human ace2 orf cdna - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "O-GlcNAcylation at S659 enhances SARS-CoV-2 spike protein stability and pseudoparticle packaging efficiency"

    Article Title: O-GlcNAcylation at S659 enhances SARS-CoV-2 spike protein stability and pseudoparticle packaging efficiency

    Journal: Microbiology Spectrum

    doi: 10.1128/spectrum.00527-25

    O-GlcNAcylation of S at S659 regulates pseudoviral packaging of SARS-CoV-2. ( A ) S-S659A does not affect the binding between S and Ace2. HEK293T cells were transfected with the plasmids expressing HA-ACE2 and GFP-S-WT, GFP-S-659A, or empty vector. The cell lysates were subject to IP. ( B ) Quantitation of ( A ). ( C ) Diagram of four-plasmid system for SARS-CoV-2pp production. The four-plasmid system for preparing pseudovirus SARS-CoV-2pp includes Luc (pLenti6) encoding luciferase, HIV Gag/pol (pLP1), HIV Rev (pLP2), and GFP-S. ( D ) S659A mutation in SARS-CoV-2 S reduced the SARS-CoV2pp package efficiency. The luciferase activity of lysates from Huh7.5.1 cells infected with SARS-CoV-2pp generated from HEK293T cells based on either S or the S-S659A was assayed. ns, no significance; ****, P < 0.0001.
    Figure Legend Snippet: O-GlcNAcylation of S at S659 regulates pseudoviral packaging of SARS-CoV-2. ( A ) S-S659A does not affect the binding between S and Ace2. HEK293T cells were transfected with the plasmids expressing HA-ACE2 and GFP-S-WT, GFP-S-659A, or empty vector. The cell lysates were subject to IP. ( B ) Quantitation of ( A ). ( C ) Diagram of four-plasmid system for SARS-CoV-2pp production. The four-plasmid system for preparing pseudovirus SARS-CoV-2pp includes Luc (pLenti6) encoding luciferase, HIV Gag/pol (pLP1), HIV Rev (pLP2), and GFP-S. ( D ) S659A mutation in SARS-CoV-2 S reduced the SARS-CoV2pp package efficiency. The luciferase activity of lysates from Huh7.5.1 cells infected with SARS-CoV-2pp generated from HEK293T cells based on either S or the S-S659A was assayed. ns, no significance; ****, P < 0.0001.

    Techniques Used: Binding Assay, Transfection, Expressing, Plasmid Preparation, Quantitation Assay, Luciferase, Mutagenesis, Activity Assay, Infection, Generated

    The model of O-GlcNAcylation of the SARS-CoV-2 S protein. The S protein is modified by O-GlcNAcylation, which is promoted by OGT and reduced by OGA. O-GlcNAcylation enhances the stability of the S protein, while non-GlcNAcylated S is targeted for ubiquitination and degradation. Thiamet G, an OGA inhibitor, elevates the levels of O-GlcNAcylation on the S protein. Proper glycosylation of the S protein is essential for the entry of SARS-CoV-2pp, which is mediated by the ACE2 receptor.
    Figure Legend Snippet: The model of O-GlcNAcylation of the SARS-CoV-2 S protein. The S protein is modified by O-GlcNAcylation, which is promoted by OGT and reduced by OGA. O-GlcNAcylation enhances the stability of the S protein, while non-GlcNAcylated S is targeted for ubiquitination and degradation. Thiamet G, an OGA inhibitor, elevates the levels of O-GlcNAcylation on the S protein. Proper glycosylation of the S protein is essential for the entry of SARS-CoV-2pp, which is mediated by the ACE2 receptor.

    Techniques Used: Modification, Ubiquitin Proteomics, Glycoproteomics

    Related Articles

    Clone Assay:

    Article Title: Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits.
    Article Snippet: .. Human ACE2 cDNA (HG10108-UT; Sino Biological, Inc., Chesterbrook, PA, USA) was cloned behind the murine Scgb1a1 (MSCV Puro-CCSP : GFP, #67487, Addgene, Watertown, MA, USA) or human SFTPB promoter (#88868, Addgene), generating lung-specific hACE2 expression vectors. ..

    Article Title: Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits
    Article Snippet: .. Human ACE2 cDNA (HG10108-UT; Sino Biological, Inc., Chesterbrook, PA, USA) was cloned behind the murine Scgb1a1 (MSCV Puro-CCSP : GFP, #67487, Addgene, Watertown, MA, USA) or human SFTPB promoter (#88868, Addgene), generating lung-specific hACE2 expression vectors. ..

    Expressing:

    Article Title: Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits.
    Article Snippet: .. Human ACE2 cDNA (HG10108-UT; Sino Biological, Inc., Chesterbrook, PA, USA) was cloned behind the murine Scgb1a1 (MSCV Puro-CCSP : GFP, #67487, Addgene, Watertown, MA, USA) or human SFTPB promoter (#88868, Addgene), generating lung-specific hACE2 expression vectors. ..

    Article Title: Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits
    Article Snippet: .. Human ACE2 cDNA (HG10108-UT; Sino Biological, Inc., Chesterbrook, PA, USA) was cloned behind the murine Scgb1a1 (MSCV Puro-CCSP : GFP, #67487, Addgene, Watertown, MA, USA) or human SFTPB promoter (#88868, Addgene), generating lung-specific hACE2 expression vectors. ..

    Stable Transfection:

    Article Title: Seven-month kinetics of SARS-CoV-2 antibodies and role of pre-existing antibodies to human coronaviruses
    Article Snippet: .. The stable cell line 300.19-ACE2 was obtained by transfecting 300.19 cells with a plasmid encoding human ACE2 cDNA (SinoBiological) with an Amaxa cell line Nucleofector kit V, followed by hygromycin selection and subsequent subcloning. .. RBDmFc fusion protein, containing RBD fused to the Fc region of murine IgG1 was obtained by cloning RBD amplified from the pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene) into the PFUSE-mIGg1-Fc1 (InvivoGen).

    Article Title: Seven-month kinetics of SARS-CoV-2 antibodies and protective role of pre-existing antibodies to seasonal human coronaviruses on COVID-19
    Article Snippet: .. The stable cell line 300.19-ACE2 was obtained by transfecting 300.19 cells with a plasmid encoding human ACE2 cDNA (SinoBiological) with an Amaxa cell line Nucleofector kit V, followed by hygromycin selection and subsequent subcloning. .. RBD-mFc fusion protein, containing RBD fused to the Fc region of murine IgG1 was obtained by cloning RBD amplified from the pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene) into the PFUSE-mIGg1-Fc1 (InvivoGen).

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 °C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation.
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 ◦C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Plasmid Preparation:

    Article Title: Seven-month kinetics of SARS-CoV-2 antibodies and role of pre-existing antibodies to human coronaviruses
    Article Snippet: .. The stable cell line 300.19-ACE2 was obtained by transfecting 300.19 cells with a plasmid encoding human ACE2 cDNA (SinoBiological) with an Amaxa cell line Nucleofector kit V, followed by hygromycin selection and subsequent subcloning. .. RBDmFc fusion protein, containing RBD fused to the Fc region of murine IgG1 was obtained by cloning RBD amplified from the pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene) into the PFUSE-mIGg1-Fc1 (InvivoGen).

    Article Title: Seven-month kinetics of SARS-CoV-2 antibodies and protective role of pre-existing antibodies to seasonal human coronaviruses on COVID-19
    Article Snippet: .. The stable cell line 300.19-ACE2 was obtained by transfecting 300.19 cells with a plasmid encoding human ACE2 cDNA (SinoBiological) with an Amaxa cell line Nucleofector kit V, followed by hygromycin selection and subsequent subcloning. .. RBD-mFc fusion protein, containing RBD fused to the Fc region of murine IgG1 was obtained by cloning RBD amplified from the pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene) into the PFUSE-mIGg1-Fc1 (InvivoGen).

    Selection:

    Article Title: Seven-month kinetics of SARS-CoV-2 antibodies and role of pre-existing antibodies to human coronaviruses
    Article Snippet: .. The stable cell line 300.19-ACE2 was obtained by transfecting 300.19 cells with a plasmid encoding human ACE2 cDNA (SinoBiological) with an Amaxa cell line Nucleofector kit V, followed by hygromycin selection and subsequent subcloning. .. RBDmFc fusion protein, containing RBD fused to the Fc region of murine IgG1 was obtained by cloning RBD amplified from the pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene) into the PFUSE-mIGg1-Fc1 (InvivoGen).

    Article Title: Seven-month kinetics of SARS-CoV-2 antibodies and protective role of pre-existing antibodies to seasonal human coronaviruses on COVID-19
    Article Snippet: .. The stable cell line 300.19-ACE2 was obtained by transfecting 300.19 cells with a plasmid encoding human ACE2 cDNA (SinoBiological) with an Amaxa cell line Nucleofector kit V, followed by hygromycin selection and subsequent subcloning. .. RBD-mFc fusion protein, containing RBD fused to the Fc region of murine IgG1 was obtained by cloning RBD amplified from the pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene) into the PFUSE-mIGg1-Fc1 (InvivoGen).

    Subcloning:

    Article Title: Seven-month kinetics of SARS-CoV-2 antibodies and role of pre-existing antibodies to human coronaviruses
    Article Snippet: .. The stable cell line 300.19-ACE2 was obtained by transfecting 300.19 cells with a plasmid encoding human ACE2 cDNA (SinoBiological) with an Amaxa cell line Nucleofector kit V, followed by hygromycin selection and subsequent subcloning. .. RBDmFc fusion protein, containing RBD fused to the Fc region of murine IgG1 was obtained by cloning RBD amplified from the pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene) into the PFUSE-mIGg1-Fc1 (InvivoGen).

    Article Title: Seven-month kinetics of SARS-CoV-2 antibodies and protective role of pre-existing antibodies to seasonal human coronaviruses on COVID-19
    Article Snippet: .. The stable cell line 300.19-ACE2 was obtained by transfecting 300.19 cells with a plasmid encoding human ACE2 cDNA (SinoBiological) with an Amaxa cell line Nucleofector kit V, followed by hygromycin selection and subsequent subcloning. .. RBD-mFc fusion protein, containing RBD fused to the Fc region of murine IgG1 was obtained by cloning RBD amplified from the pcDNA3-SARS-CoV-2-S-RBD-Fc (Addgene) into the PFUSE-mIGg1-Fc1 (InvivoGen).

    Transfection:

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 °C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation.
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 ◦C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Flow Cytometry:

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 °C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation.
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 ◦C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Incubation:

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 °C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation.
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 ◦C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Produced:

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 °C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.

    Article Title: Rapid High-Yield Production of Functional SARS-CoV-2 Receptor Binding Domain by Viral and Non-Viral Transient Expression for Pre-Clinical Evaluation.
    Article Snippet: .. HEK293 cells stably transfected with human ACE2 cDNA (Sino Biological, Wayne, PA, USA) were resuspended in 0.1 mL of flow cytometry buffer and incubated with the test proteins (RBD, to which a His-Tag was engineered at the N-terminal, produced by two manufacturing processes) or a standard S1 reference protein at 0.25 and 0.5 μg/mL for 20 min at 4 ◦C. .. The RBD samples from the transfection and infection approaches used were those from the final elutions in which RBD was detected with a purity over 95% by silver staining of SDS-PAGE gels.



    Similar Products

    human ace2 orf cdna  (Sino Biological)
    96
    Sino Biological human ace2 orf cdna
    O-GlcNAcylation of S at S659 regulates pseudoviral packaging of SARS-CoV-2. ( A ) S-S659A does not affect the binding between S and <t>Ace2.</t> HEK293T cells were transfected with the plasmids expressing HA-ACE2 and GFP-S-WT, GFP-S-659A, or empty vector. The cell lysates were subject to IP. ( B ) Quantitation of ( A ). ( C ) Diagram of four-plasmid system for SARS-CoV-2pp production. The four-plasmid system for preparing pseudovirus SARS-CoV-2pp includes Luc (pLenti6) encoding luciferase, HIV Gag/pol (pLP1), HIV Rev (pLP2), and GFP-S. ( D ) S659A mutation in SARS-CoV-2 S reduced the SARS-CoV2pp package efficiency. The luciferase activity of lysates from Huh7.5.1 cells infected with SARS-CoV-2pp generated from HEK293T cells based on either S or the S-S659A was assayed. ns, no significance; ****, P < 0.0001.
    Human Ace2 Orf Cdna, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+cdna/Human+ACE2+%2F+Angiotensin-Converting+Enzyme+2+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-HA+tag/pmc12403561-103-1-15
    Average 96 stars, based on 1 article reviews
    human ace2 orf cdna - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    human ace2 cdna  (OriGene)
    94
    OriGene human ace2 cdna
    Relative mRNA detection and western blot analysis of Human <t>ACE2</t> expression. Kidney (K), lung (L) and brain (B) tissues were collected from the indicated transgenic hemizygous (HEM); homozygous (HOM) and wild type (WT) rats. (A,D) F344-Tg(CAG- ACE2 )057Bryd; (B,E) SD-Tg( ACE2 )955CPBryd; (C,F) SD-Tg( ACE2 )058CVBryd line. (A–C) RT-PCR analysis. The rat reference gene Hprt1 was used to normalize human ACE2 mRNA expression; each bar represents expression for an individual rat. Error bars represent the standard deviations between triplicate technical replicates. (D–F) Western blot analysis., HepG2 cells (PC) were used as a positive control and Rat Hprt was used as the loading control. M is the molecular size standard lane with the sizes of the standards indicated in kDa.
    Human Ace2 Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+cdna/ACE2+(NM_021804)+Human+Tagged+ORF+Clone/pmc12605091-32-1-7
    Average 94 stars, based on 1 article reviews
    human ace2 cdna - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    c ha tag  (Sino Biological)
    96
    Sino Biological c ha tag
    Relative mRNA detection and western blot analysis of Human <t>ACE2</t> expression. Kidney (K), lung (L) and brain (B) tissues were collected from the indicated transgenic hemizygous (HEM); homozygous (HOM) and wild type (WT) rats. (A,D) F344-Tg(CAG- ACE2 )057Bryd; (B,E) SD-Tg( ACE2 )955CPBryd; (C,F) SD-Tg( ACE2 )058CVBryd line. (A–C) RT-PCR analysis. The rat reference gene Hprt1 was used to normalize human ACE2 mRNA expression; each bar represents expression for an individual rat. Error bars represent the standard deviations between triplicate technical replicates. (D–F) Western blot analysis., HepG2 cells (PC) were used as a positive control and Rat Hprt was used as the loading control. M is the molecular size standard lane with the sizes of the standards indicated in kDa.
    C Ha Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+cdna/Human+ACE2+%2F+Angiotensin-Converting+Enzyme+2+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-HA+tag/pmc12403561-103-10-15
    Average 96 stars, based on 1 article reviews
    c ha tag - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    enzyme 2  (Sino Biological)
    96
    Sino Biological enzyme 2
    Relative mRNA detection and western blot analysis of Human <t>ACE2</t> expression. Kidney (K), lung (L) and brain (B) tissues were collected from the indicated transgenic hemizygous (HEM); homozygous (HOM) and wild type (WT) rats. (A,D) F344-Tg(CAG- ACE2 )057Bryd; (B,E) SD-Tg( ACE2 )955CPBryd; (C,F) SD-Tg( ACE2 )058CVBryd line. (A–C) RT-PCR analysis. The rat reference gene Hprt1 was used to normalize human ACE2 mRNA expression; each bar represents expression for an individual rat. Error bars represent the standard deviations between triplicate technical replicates. (D–F) Western blot analysis., HepG2 cells (PC) were used as a positive control and Rat Hprt was used as the loading control. M is the molecular size standard lane with the sizes of the standards indicated in kDa.
    Enzyme 2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+cdna/Human+ACE2+%2F+Angiotensin-Converting+Enzyme+2+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-His+tag/pmc12264959-137-7-10
    Average 96 stars, based on 1 article reviews
    enzyme 2 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    plasmid pcmv3 hace2 expressing wt hace2  (Sino Biological)
    96
    Sino Biological plasmid pcmv3 hace2 expressing wt hace2
    Relative mRNA detection and western blot analysis of Human <t>ACE2</t> expression. Kidney (K), lung (L) and brain (B) tissues were collected from the indicated transgenic hemizygous (HEM); homozygous (HOM) and wild type (WT) rats. (A,D) F344-Tg(CAG- ACE2 )057Bryd; (B,E) SD-Tg( ACE2 )955CPBryd; (C,F) SD-Tg( ACE2 )058CVBryd line. (A–C) RT-PCR analysis. The rat reference gene Hprt1 was used to normalize human ACE2 mRNA expression; each bar represents expression for an individual rat. Error bars represent the standard deviations between triplicate technical replicates. (D–F) Western blot analysis., HepG2 cells (PC) were used as a positive control and Rat Hprt was used as the loading control. M is the molecular size standard lane with the sizes of the standards indicated in kDa.
    Plasmid Pcmv3 Hace2 Expressing Wt Hace2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+cdna/Human+ACE2+%2F+Angiotensin-Converting+Enzyme+2+Gene+ORF+cDNA+clone+expression+plasmid%2C+N-Flag+tag/10__1016_slash_j__celbio__2025__100017-114-9-19
    Average 96 stars, based on 1 article reviews
    plasmid pcmv3 hace2 expressing wt hace2 - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    plasmids  (Sino Biological)
    96
    Sino Biological plasmids
    Relative mRNA detection and western blot analysis of Human <t>ACE2</t> expression. Kidney (K), lung (L) and brain (B) tissues were collected from the indicated transgenic hemizygous (HEM); homozygous (HOM) and wild type (WT) rats. (A,D) F344-Tg(CAG- ACE2 )057Bryd; (B,E) SD-Tg( ACE2 )955CPBryd; (C,F) SD-Tg( ACE2 )058CVBryd line. (A–C) RT-PCR analysis. The rat reference gene Hprt1 was used to normalize human ACE2 mRNA expression; each bar represents expression for an individual rat. Error bars represent the standard deviations between triplicate technical replicates. (D–F) Western blot analysis., HepG2 cells (PC) were used as a positive control and Rat Hprt was used as the loading control. M is the molecular size standard lane with the sizes of the standards indicated in kDa.
    Plasmids, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+ace2+cdna/Human+ACE2+%2F+Angiotensin-Converting+Enzyme+2+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-His+tag/pm39856746-113-5-10
    Average 96 stars, based on 1 article reviews
    plasmids - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    O-GlcNAcylation of S at S659 regulates pseudoviral packaging of SARS-CoV-2. ( A ) S-S659A does not affect the binding between S and Ace2. HEK293T cells were transfected with the plasmids expressing HA-ACE2 and GFP-S-WT, GFP-S-659A, or empty vector. The cell lysates were subject to IP. ( B ) Quantitation of ( A ). ( C ) Diagram of four-plasmid system for SARS-CoV-2pp production. The four-plasmid system for preparing pseudovirus SARS-CoV-2pp includes Luc (pLenti6) encoding luciferase, HIV Gag/pol (pLP1), HIV Rev (pLP2), and GFP-S. ( D ) S659A mutation in SARS-CoV-2 S reduced the SARS-CoV2pp package efficiency. The luciferase activity of lysates from Huh7.5.1 cells infected with SARS-CoV-2pp generated from HEK293T cells based on either S or the S-S659A was assayed. ns, no significance; ****, P < 0.0001.

    Journal: Microbiology Spectrum

    Article Title: O-GlcNAcylation at S659 enhances SARS-CoV-2 spike protein stability and pseudoparticle packaging efficiency

    doi: 10.1128/spectrum.00527-25

    Figure Lengend Snippet: O-GlcNAcylation of S at S659 regulates pseudoviral packaging of SARS-CoV-2. ( A ) S-S659A does not affect the binding between S and Ace2. HEK293T cells were transfected with the plasmids expressing HA-ACE2 and GFP-S-WT, GFP-S-659A, or empty vector. The cell lysates were subject to IP. ( B ) Quantitation of ( A ). ( C ) Diagram of four-plasmid system for SARS-CoV-2pp production. The four-plasmid system for preparing pseudovirus SARS-CoV-2pp includes Luc (pLenti6) encoding luciferase, HIV Gag/pol (pLP1), HIV Rev (pLP2), and GFP-S. ( D ) S659A mutation in SARS-CoV-2 S reduced the SARS-CoV2pp package efficiency. The luciferase activity of lysates from Huh7.5.1 cells infected with SARS-CoV-2pp generated from HEK293T cells based on either S or the S-S659A was assayed. ns, no significance; ****, P < 0.0001.

    Article Snippet: The human ACE2 ORF cDNA clone expression plasmid with a C-HA tag was sourced from Sino Biological (HG10108-CY).

    Techniques: Binding Assay, Transfection, Expressing, Plasmid Preparation, Quantitation Assay, Luciferase, Mutagenesis, Activity Assay, Infection, Generated

    The model of O-GlcNAcylation of the SARS-CoV-2 S protein. The S protein is modified by O-GlcNAcylation, which is promoted by OGT and reduced by OGA. O-GlcNAcylation enhances the stability of the S protein, while non-GlcNAcylated S is targeted for ubiquitination and degradation. Thiamet G, an OGA inhibitor, elevates the levels of O-GlcNAcylation on the S protein. Proper glycosylation of the S protein is essential for the entry of SARS-CoV-2pp, which is mediated by the ACE2 receptor.

    Journal: Microbiology Spectrum

    Article Title: O-GlcNAcylation at S659 enhances SARS-CoV-2 spike protein stability and pseudoparticle packaging efficiency

    doi: 10.1128/spectrum.00527-25

    Figure Lengend Snippet: The model of O-GlcNAcylation of the SARS-CoV-2 S protein. The S protein is modified by O-GlcNAcylation, which is promoted by OGT and reduced by OGA. O-GlcNAcylation enhances the stability of the S protein, while non-GlcNAcylated S is targeted for ubiquitination and degradation. Thiamet G, an OGA inhibitor, elevates the levels of O-GlcNAcylation on the S protein. Proper glycosylation of the S protein is essential for the entry of SARS-CoV-2pp, which is mediated by the ACE2 receptor.

    Article Snippet: The human ACE2 ORF cDNA clone expression plasmid with a C-HA tag was sourced from Sino Biological (HG10108-CY).

    Techniques: Modification, Ubiquitin Proteomics, Glycoproteomics

    Relative mRNA detection and western blot analysis of Human ACE2 expression. Kidney (K), lung (L) and brain (B) tissues were collected from the indicated transgenic hemizygous (HEM); homozygous (HOM) and wild type (WT) rats. (A,D) F344-Tg(CAG- ACE2 )057Bryd; (B,E) SD-Tg( ACE2 )955CPBryd; (C,F) SD-Tg( ACE2 )058CVBryd line. (A–C) RT-PCR analysis. The rat reference gene Hprt1 was used to normalize human ACE2 mRNA expression; each bar represents expression for an individual rat. Error bars represent the standard deviations between triplicate technical replicates. (D–F) Western blot analysis., HepG2 cells (PC) were used as a positive control and Rat Hprt was used as the loading control. M is the molecular size standard lane with the sizes of the standards indicated in kDa.

    Journal: Frontiers in Microbiology

    Article Title: Generation and characterization of a humanized ACE2 rat model for the study of SARS-CoV-2 and COVID-19

    doi: 10.3389/fmicb.2025.1680273

    Figure Lengend Snippet: Relative mRNA detection and western blot analysis of Human ACE2 expression. Kidney (K), lung (L) and brain (B) tissues were collected from the indicated transgenic hemizygous (HEM); homozygous (HOM) and wild type (WT) rats. (A,D) F344-Tg(CAG- ACE2 )057Bryd; (B,E) SD-Tg( ACE2 )955CPBryd; (C,F) SD-Tg( ACE2 )058CVBryd line. (A–C) RT-PCR analysis. The rat reference gene Hprt1 was used to normalize human ACE2 mRNA expression; each bar represents expression for an individual rat. Error bars represent the standard deviations between triplicate technical replicates. (D–F) Western blot analysis., HepG2 cells (PC) were used as a positive control and Rat Hprt was used as the loading control. M is the molecular size standard lane with the sizes of the standards indicated in kDa.

    Article Snippet: The human ACE2 cDNA was purchased from Origene (Catalog #RG208442) and subcloned into pCAG-loxP-STOP-loxP-ZsGreen (Addgene plasmid #51269) using restriction enzymes Sal I and Sac I.

    Techniques: Western Blot, Expressing, Transgenic Assay, Reverse Transcription Polymerase Chain Reaction, Positive Control, Control

    F344-Tg(CAG- ACE2 )057Bryd transgenic rats are susceptible to SARS-CoV-2 challenge. Groups of F344-Tg(CAG- ACE2 )057Bryd littermates without the ACE2 transgene (wild type, WT, orange circles), or hemizygous (HEMI, blue squares) or homozygous (HOM, purple triangles) for transgene expression were challenge with SARS-CoV-2 by intranasal instillation of 14 PFU of the USA-WA1/2020 variant. Rats were monitored for survival (A) and clinical symptoms including weight loss (B) for up to 10 days. Weight loss of hemizygous animals has been separated into those that survived (solid lines, n = 2) and those that succumbed to disease (dashed lines, n = 10). Animals identified as moribund were humanely euthanized, and tissues were collected for histological analysis (see , ). N = 7 (WT), 13 (HEMI), and 6 (HOM) collected in two independent experiments. Survival data was analyzed by Mantel-Cox (log-rank) test. Daily change in weight was analyzed by ANOVA with Dunn’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001 relative to WT. Survival of hemizygous and homozygous groups were not significantly different from each other.

    Journal: Frontiers in Microbiology

    Article Title: Generation and characterization of a humanized ACE2 rat model for the study of SARS-CoV-2 and COVID-19

    doi: 10.3389/fmicb.2025.1680273

    Figure Lengend Snippet: F344-Tg(CAG- ACE2 )057Bryd transgenic rats are susceptible to SARS-CoV-2 challenge. Groups of F344-Tg(CAG- ACE2 )057Bryd littermates without the ACE2 transgene (wild type, WT, orange circles), or hemizygous (HEMI, blue squares) or homozygous (HOM, purple triangles) for transgene expression were challenge with SARS-CoV-2 by intranasal instillation of 14 PFU of the USA-WA1/2020 variant. Rats were monitored for survival (A) and clinical symptoms including weight loss (B) for up to 10 days. Weight loss of hemizygous animals has been separated into those that survived (solid lines, n = 2) and those that succumbed to disease (dashed lines, n = 10). Animals identified as moribund were humanely euthanized, and tissues were collected for histological analysis (see , ). N = 7 (WT), 13 (HEMI), and 6 (HOM) collected in two independent experiments. Survival data was analyzed by Mantel-Cox (log-rank) test. Daily change in weight was analyzed by ANOVA with Dunn’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001 relative to WT. Survival of hemizygous and homozygous groups were not significantly different from each other.

    Article Snippet: The human ACE2 cDNA was purchased from Origene (Catalog #RG208442) and subcloned into pCAG-loxP-STOP-loxP-ZsGreen (Addgene plasmid #51269) using restriction enzymes Sal I and Sac I.

    Techniques: Transgenic Assay, Expressing, Variant Assay, Comparison

    Tissue damage of F344-Tg(CAG- ACE2 )057Bryd rats challenged with SARS-CoV-2. Sections of lung (A,D) , eye (B,E) and nasal cavity (C,F) from wild type (A–C) and F344-Tg(CAG- ACE 2)057Bryd hemizygous rats infected with either high (D) or low (E,F) doses of SARS-CoV-2 stained with hematoxylin and eosin. (D) One hemizygous rat infected with the high dose demonstrates broncho and interstitial pneumonia. Bronchiolar lumina are multifocally filled with mucinous material and low numbers of neutrophils; alveolar septae and lumina contain alveolar macrophages, lymphocytes and mild hemorrhage. (E) Hemizygous rats infected with the low dose show anterior uveitis characterized by infiltration of the iris and choroid body with neutrophils and lymphocytes and anterior chamber accumulations of eosinophilic flocculent material, neutrophils, few lymphocytes and red blood cells. The corneal stroma is infiltrated by moderate numbers of neutrophils. (F) Hemizygous rats infected with the low dose show foci of epithelial erosion and associated mucosal neutrophilic infiltrates as well as accumulation of suppurative exudate in the nasal cavity. All images taken at 200× magnification.

    Journal: Frontiers in Microbiology

    Article Title: Generation and characterization of a humanized ACE2 rat model for the study of SARS-CoV-2 and COVID-19

    doi: 10.3389/fmicb.2025.1680273

    Figure Lengend Snippet: Tissue damage of F344-Tg(CAG- ACE2 )057Bryd rats challenged with SARS-CoV-2. Sections of lung (A,D) , eye (B,E) and nasal cavity (C,F) from wild type (A–C) and F344-Tg(CAG- ACE 2)057Bryd hemizygous rats infected with either high (D) or low (E,F) doses of SARS-CoV-2 stained with hematoxylin and eosin. (D) One hemizygous rat infected with the high dose demonstrates broncho and interstitial pneumonia. Bronchiolar lumina are multifocally filled with mucinous material and low numbers of neutrophils; alveolar septae and lumina contain alveolar macrophages, lymphocytes and mild hemorrhage. (E) Hemizygous rats infected with the low dose show anterior uveitis characterized by infiltration of the iris and choroid body with neutrophils and lymphocytes and anterior chamber accumulations of eosinophilic flocculent material, neutrophils, few lymphocytes and red blood cells. The corneal stroma is infiltrated by moderate numbers of neutrophils. (F) Hemizygous rats infected with the low dose show foci of epithelial erosion and associated mucosal neutrophilic infiltrates as well as accumulation of suppurative exudate in the nasal cavity. All images taken at 200× magnification.

    Article Snippet: The human ACE2 cDNA was purchased from Origene (Catalog #RG208442) and subcloned into pCAG-loxP-STOP-loxP-ZsGreen (Addgene plasmid #51269) using restriction enzymes Sal I and Sac I.

    Techniques: Infection, Staining

    Cellular changes in the brains of F344-Tg(CAG- ACE2 )057Bryd rats that succumbed to a high dose SARS-CoV-2 challenge. Sections of cerebrum and cerebellum from wild type (A–D) and F344-Tg(CAG- ACE2 )057Bryd hemizygous rats (E–H) infected with SARS-CoV-2 stained with hematoxylin and eosin (A,D,E,H) or subjected to IBA (B,F) or GFAP (C,G) immunohistochemistry. (E) Histology of hemizygous rats exhibit macrophagic meningitis, perivascular cuffing, encephalitis and necrosis. (F) IBA IHC demonstrates marked glial cell activation. (G) GFAP IHC shows astrocyte proliferation swelling in areas of gliosis. (H) Cerebellum exhibits Purkinje cell loss associated with vacuolated neuropil of the adjacent molecular layer and scattered pyknotic and karyorrhectic debris (apoptosis as determined by PCNA IHC) with mildly increased numbers of astrocytes or microglia. All images taken at 200× magnification.

    Journal: Frontiers in Microbiology

    Article Title: Generation and characterization of a humanized ACE2 rat model for the study of SARS-CoV-2 and COVID-19

    doi: 10.3389/fmicb.2025.1680273

    Figure Lengend Snippet: Cellular changes in the brains of F344-Tg(CAG- ACE2 )057Bryd rats that succumbed to a high dose SARS-CoV-2 challenge. Sections of cerebrum and cerebellum from wild type (A–D) and F344-Tg(CAG- ACE2 )057Bryd hemizygous rats (E–H) infected with SARS-CoV-2 stained with hematoxylin and eosin (A,D,E,H) or subjected to IBA (B,F) or GFAP (C,G) immunohistochemistry. (E) Histology of hemizygous rats exhibit macrophagic meningitis, perivascular cuffing, encephalitis and necrosis. (F) IBA IHC demonstrates marked glial cell activation. (G) GFAP IHC shows astrocyte proliferation swelling in areas of gliosis. (H) Cerebellum exhibits Purkinje cell loss associated with vacuolated neuropil of the adjacent molecular layer and scattered pyknotic and karyorrhectic debris (apoptosis as determined by PCNA IHC) with mildly increased numbers of astrocytes or microglia. All images taken at 200× magnification.

    Article Snippet: The human ACE2 cDNA was purchased from Origene (Catalog #RG208442) and subcloned into pCAG-loxP-STOP-loxP-ZsGreen (Addgene plasmid #51269) using restriction enzymes Sal I and Sac I.

    Techniques: Infection, Staining, Immunohistochemistry, Activation Assay